Video 13. Pollen tube reception defect in a lre-5/lre-5 ovule is recapitulated in an in vitro guidance assay

Time-lapse imaging of LAT52:DsRed-tagged pollen tube entering a lre-5/lre-5 ovule, reaching DD3:GFP-marked synergid cell, continuing to grow incessantly in the synergid cell and eventually growing further into the female gametophyte.

The movie shows merged time-lapse images captured in red and green channels.

Time elapsed between two frames is 10 minutes.

Ref: Tsukamoto, T., Qin Y., Huang, Y., Dunatunga, D. and Palanivelu, R. (2010). Plant J (in press).